SWITCH IN GAP JUNCTION PROTEIN EXPRESSION IS ASSOCIATED WITH SELECTIVE CHANGES IN JUNCTIONAL PERMEABILITY DURING KERATINOCYTE DIFFERENTIATION

被引:132
作者
BRISSETTE, JL
KUMAR, NM
GILULA, NB
HALL, JE
DOTTO, GP
机构
[1] HARVARD UNIV, SCH MED, DEPT DERMATOL, BOSTON, MA 02129 USA
[2] MASSACHUSETTS GEN HOSP E, CUTANEOUS BIOL RES CTR, BOSTON, MA 02129 USA
[3] Scripps Res Inst, DEPT CELL BIOL, LA JOLLA, CA 92037 USA
[4] UNIV CALIF IRVINE, DEPT PHYSIOL & BIOPHYS, IRVINE, CA 92717 USA
关键词
MOUSE PRIMARY KERATINOCYTES; CALCIUM; CONNEXINS; CHANNELS;
D O I
10.1073/pnas.91.14.6453
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Gap junctional communication provides a mechanism for regulating multicellular activities by allowing the exchange of small diffusible molecules between neighboring cells. The diversity of gap junction proteins may exist to form channels that have different permeability properties. We report here that induction of terminal differentiation in mouse primary keratinocytes by calcium results in a specific switch in gap junction protein expression. Expression of alpha(1) (connexin 43) and beta(2) (connexin 26) gap junction proteins is down-modulated, whereas that of beta(3) (connexin 31) and beta(4) (connexin 31.1) proteins is induced. Although both proliferating and differentiating keratinocytes are electrically coupled, there are significant changes in the permeability properties of the junctions to small molecules. In parallel with the changes in gap junction protein expression during differentiation, the intercellular transfer of the small dyes neurobiotin, carboxyfluorescein, and Lucifer yellow is significantly reduced, whereas that of small metabolites, such as nucleotides and amino acids, proceeds unimpeded. Thus, a switch in gap junction protein expression in differentiating keratinocytes is accompanied by selective changes in junctional permeability that may play an important role in the coordinate control of the differentiation process.
引用
收藏
页码:6453 / 6457
页数:5
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