THE LARGE NONSYMBIOTIC PLASMID PRMEGR4A OF RHIZOBIUM-MELILOTI GR4 ENCODES A PROTEIN INVOLVED IN REPLICATION THAT HAS HOMOLOGY WITH THE REPC PROTEIN OF AGROBACTERIUM PLASMIDS

被引：26

作者：

MERCADOBLANCO, J ^{[1
]}

OLIVARES, J ^{[1
]}

机构：

[1] CSIC,DEPT MICROBIOL,ESTAC EXPT ZAIDIN,E-18008 GRANADA,SPAIN

来源：

PLASMID | 1994年 / 32卷 / 01期

关键词：

D O I：

10.1006/plas.1994.1046

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The large plasmid, pRmeGR4a, of Rhizobium meliloti GR4 is a nonsymbiotic, self-transmissible replicon that shows a high degree of stability. Plasmid replication and stabilization functions are located on a 4.8-kb PstI fragment. Analysis of the nucleotide sequence of this DNA region revealed the presence of six open reading frames (ORFs) with coding capabilities; all are transcribed in the same direction. Only one of the ORFs (ORF3) seems to be essential for replication. Its predicted protein sequence shows homology with RepC, a protein that has been suggested to be involved in the replication of Agrobacterium Ri and Ti plasmids. (C) 1994 Academic Press, Inc.

引用

页码：75 / 79

页数：5

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