THE HUMAN BETA-GLOBIN GENE CONTAINS MULTIPLE REGULATORY REGIONS - IDENTIFICATION OF ONE PROMOTER AND 2 DOWNSTREAM ENHANCERS

被引：165

作者：

ANTONIOU, M ^{[1
]}

DEBOER, E ^{[1
]}

HABETS, G ^{[1
]}

GROSVELD, F ^{[1
]}

机构：

[1] NATL INST MED RES, GENE STRUCT & EXPRESS LAB, RIDGEWAY, MILL HILL, LONDON NW7 1AA, ENGLAND

来源：

EMBO JOURNAL | 1988年 / 7卷 / 02期

关键词：

D O I：

10.1002/j.1460-2075.1988.tb02824.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have introduced into murine erythroleukaemia (MEL) cells several series of deletion mutants of hybrid genes between the human .beta.-globin gene and the murine H-2Kb gene. S1 nuclease and transcriptional run-off analysis showed that the human .beta.-globin gene contains at least three globin specific transcriptional control elements. One is a promoter element and is located 160 bp upstream from the transcriptional initiation site; it increases the efficiency of the promoter after MEL cells are induces to differentiate. The other two elements are tissue-specific transcriptional enhancers and are located downstream from the transcriptional initiation site, the first in the structural .beta.-globin gene and the second in the 3'' flanking sequences.

引用

页码：377 / 384

页数：8

共 35 条

[1] K562 - HUMAN ERYTHROLEUKEMIC CELL-LINE [J].

ANDERSSON, LC ;

NILSSON, K ;

GAHMBERG, CG .

INTERNATIONAL JOURNAL OF CANCER, 1979, 23 (02) :143-147

[2]

ANTONIOU M, 1987, NUCLEIC ACIDS RES, V15, P1886

[3]

ANTONIOU M, 1986, HUMAN GENETIC DISEAS, P65

[4] THE ROLE OF THE KAPPA-ENHANCER AND ITS BINDING-FACTOR NF-KAPPA-B IN THE DEVELOPMENTAL REGULATION OF KAPPA-GENE TRANSCRIPTION [J].

ATCHISON, ML ;

PERRY, RP .

CELL, 1987, 48 (01) :121-128

[5] MUTUALLY EXCLUSIVE INTERACTION OF THE CCAAT-BINDING FACTOR AND OF A DISPLACEMENT PROTEIN WITH OVERLAPPING SEQUENCES OF A HISTONE GENE PROMOTER [J].

BARBERIS, A ;

SUPERTIFURGA, G ;

BUSSLINGER, M .

CELL, 1987, 50 (03) :347-359

[6] 2 3' SEQUENCES DIRECT ADULT ERYTHROID-SPECIFIC EXPRESSION OF HUMAN BETA-GLOBIN GENES IN TRANSGENIC MICE [J].

BEHRINGER, RR ;

HAMMER, RE ;

BRINSTER, RL ;

PALMITER, RD ;

TOWNES, TM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) :7056-7060

[7] MECHANISMS THAT REGULATE IMMUNOGLOBULIN GENE-EXPRESSION [J].

CALAME, KL .

ANNUAL REVIEW OF IMMUNOLOGY, 1985, 3 :159-195

[8] SPECIFIC EXPRESSION OF A FOREIGN BETA-GLOBIN GENE IN ERYTHROID-CELLS OF TRANSGENIC MICE [J].

CHADA, K ;

MAGRAM, J ;

RAPHAEL, K ;

RADICE, G ;

LACY, E ;

COSTANTINI, F .

NATURE, 1985, 314 (6009) :377-380

[9] THE REGULATED EXPRESSION OF BETA-GLOBIN GENES INTRODUCED INTO MOUSE ERYTHROLEUKEMIA-CELLS [J].

CHAO, MV ;

MELLON, P ;

CHARNAY, P ;

MANIATIS, T ;

AXEL, R .

CELL, 1983, 32 (02) :483-493

[10] DIFFERENCES IN HUMAN ALPHA-GLOBIN AND BETA-GLOBIN GENE-EXPRESSION IN MOUSE ERYTHROLEUKEMIA-CELLS - THE ROLE OF INTRAGENIC SEQUENCES [J].

CHARNAY, P ;

TREISMAN, R ;

MELLON, P ;

CHAO, M ;

AXEL, R ;

MANIATIS, T .

CELL, 1984, 38 (01) :251-263

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