LIGHT AND ELECTRON-MICROSCOPE IMMUNOCYTOCHEMICAL LOCALIZATION OF 5-LIPOXYGENASE AND 12-LIPOXYGENASE AND CYCLOOXYGENASE ENZYMES IN HUMAN GRANULOSA-CELLS FROM PREOVULATORY FOLLICLES

被引：15

作者：

PRIDHAM, D ^{[1
]}

LEI, ZM ^{[1
]}

CHEGINI, N ^{[1
]}

RAO, CV ^{[1
]}

YUSSMAN, MA ^{[1
]}

COOK, CL ^{[1
]}

机构：

[1] UNIV LOUISVILLE,SCH MED,DEPT OBSTET & GYNECOL,LOUISVILLE,KY 40292

来源：

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1990年 / 39卷 / 03期

关键词：

D O I：

10.1016/0952-3278(90)90078-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cellular and subcellular distribution of 5- and 12-lipoxygenases and cyclooxygenase enzymes were investigated in human granulosa cells from preovulatory follicles using light and electron microscope immunocytochemistry. The results demonstrated that all three enzymes are present in granulosa cells but not in minor contaminating red blood cells. While the distribution of cyclooxygenase and 12-lipoxygenase was relatively uniform among the granulosa cells, 5-lipoxygenase was not uniformly distributed among these cells. All three enzymes are present in microvillus plasma membranes, rough endoplasmic reticulum, cytoplasm, nuclear membranes and chromatin. In summary, 5- and 12-lipoxygenases and cyclooxygenase enzymes, which catalyze the transformation of arachidonic acid into different eicosanoids, are present in several subcellular organelles including nuclei of granulosa cells from preovulatory follicles. © 1990.

引用

页码：231 / 238

页数：8

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