ULTRASTRUCTURAL-CHANGES IN MEMBRANES AND ACROSOME OF HUMAN SPERM DURING CRYOPRESERVATION

被引：55

作者：

BARTHELEMY, C ^{[1
]}

ROYERE, D ^{[1
]}

HAMMAHAH, S ^{[1
]}

LEBOS, C ^{[1
]}

THARANNE, MJ ^{[1
]}

LANSAC, J ^{[1
]}

机构：

[1] CHU BRETONNEAU,UNITE REPROD CECOS FIV,F-37044 TOURS,FRANCE

来源：

ARCHIVES OF ANDROLOGY | 1990年 / 25卷 / 01期

关键词：

Acrosome; Cryoprotective medium; Fine structure; Freezing/thawing; Human spermatozoa; Plasma membrane;

D O I：

10.3109/01485019008987591

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

Functional ultrastructural changes in human sperm heads were evaluated following semen dilution in cryoprotective medium (with and without seminal plasma) as well as freezing/thawing. Plasma membranes were as much altered by dilution as by freezing/thawing when compared with fresh samples. The most striking effect on acrosome noted during freezing/thawing was a dramatic decline in percentage of intact spermatozoa. Acrosomal changes seemed to be less important in the fractions without seminal plasma. These deleterious effects were very demonstrable using transmission electron microscopy (TEM), rather than conventional staining. TEM is useful in obtaining more detailed information on membrane and acrosome integrity until a specific procedure to evaluate functional/physical integrity can be found. Cyropreservation in the absence of seminal plasma can be used for intrauterine insemination in AIH, AID programs with frozen semen. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：29 / 40

页数：12

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