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INTERNUCLEOTIDE PHOSPHITE SULFURIZATION WITH TETRAETHYLTHIURAM DISULFIDE - PHOSPHOROTHIOATE OLIGONUCLEOTIDE SYNTHESIS VIA PHOSPHORAMIDITE CHEMISTRY

被引：139
作者：
VU, H
HIRSCHBEIN, BL
机构：
[1] Applied Bioaystems Inc., Foster City, CA 94404
来源：
TETRAHEDRON LETTERS | 1991年 / 32卷 / 26期
关键词：
D O I：
10.1016/0040-4039(91)80672-S
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A new sulfurizing reagent has been developed for the automated synthesis of phosphorothioate oligonucleotides 1 via the phosphoramidite method. Tetraethylthiuram disulfide (TETD) converts internucleotide cyanoethyl phosphite 2 to the phosphorothioate triester 3 within 15 minutes at room temperature after each coupling step. With an optimized synthesis cycle, the efficiency of phosphorothioate oligonucleotide synthesis is comparable to that of phosphodiester oligonucleotides. Large scale synthesis (200-mu-mole), oligonucleotides up to 100 bases long, and mixed phosphorothioate/phosphodiester DNA are demonstrated.
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页码：3005 / 3008
页数：4
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