A YEAST SPLICING FACTOR IS LOCALIZED IN DISCRETE SUBNUCLEAR DOMAINS

被引：25

作者：

ELLIOTT, DJ

BOWMAN, DS

ABOVICH, N

FAY, FS

ROSBASH, M

机构：

[1] BRANDEIS UNIV, DEPT BIOL, WALTHAM, MA 02254 USA

[2] UNIV MASSACHUSETTS, SCH MED, PROGRAM MOLEC BIOL, BIOMED IMAGING GRP, WORCESTER, MA 01605 USA

来源：

EMBO JOURNAL | 1992年 / 11卷 / 10期

关键词：

DIGITAL IMAGING MICROSCOPY; NUCLEAR ORGANIZATION; PRE-MESSENGER-RNA SPLICING; YEAST;

D O I：

10.1002/j.1460-2075.1992.tb05458.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Digital imaging microscopy has been used to visualize the splicing protein PRP6p and three other yeast nuclear proteins. The results show that PRP6p is uniquely localized to discrete subnuclear regions. A combination of cytological and biochemical assays suggests that these sites can be saturated when the protein is overexpressed and likely correspond to the location of U4/U6 snRNPs. The observations indicate that some splicing components are located in discrete subregions of the yeast nucleus, similar to the situation described for the mammalian nucleus.

引用

页码：3731 / 3736

页数：6

共 50 条

[1] THE YEAST PRP6 GENE ENCODES A U4/U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE (SNRNP) PROTEIN, AND THE PRP9 GENE ENCODES A PROTEIN REQUIRED FOR U2 SNRNP BINDING
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[5] MAMMALIAN NUCLEI CONTAIN FOCI WHICH ARE HIGHLY ENRICHED IN COMPONENTS OF THE PRE-MESSENGER-RNA SPLICING MACHINERY
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[6] TRANSCRIPTION-DEPENDENT COLOCALIZATION OF THE U1, U2, U4/U6, AND U5 SNRNPS IN COILED BODIES
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[J]. JOURNAL OF CELL BIOLOGY, 1992, 117 (01) : 1 - 14
[7] CARRINGTON WA, 1990, NONINVASIVE TECHNIQU, P53
[8] DISCRETE NUCLEAR DOMAINS OF POLY(A) RNA AND THEIR RELATIONSHIP TO THE FUNCTIONAL-ORGANIZATION OF THE NUCLEUS
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[J]. JOURNAL OF CELL BIOLOGY, 1991, 115 (05) : 1191 - 1202
[9] RNA11 PROTEIN IS ASSOCIATED WITH THE YEAST SPLICEOSOME AND IS LOCALIZED IN THE PERIPHERY OF THE CELL-NUCLEUS
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