CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES ON A SOLUBLE ESCHERICHIA-COLI QUINONE OXIDOREDUCTASE

被引：10

作者：

EDWARDS, KJ

THORN, JM

DANIHER, JA

DIXON, NE

OLLIS, DL

机构：

[1] Centre for Molecular Structure and Function, Research School of Chemistry, Australian National University, Canberra

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 240卷 / 05期

关键词：

QUINONE OXIDOREDUCTASE; ZETA-CRYSTALLIN; ESCHERICHIA COLI CRYSTALLIZATION; X-RAY CRYSTALLOGRAPHY;

D O I：

10.1006/jmbi.1994.1463

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single crystals suitable for X-ray diffraction studies have been obtained from a soluble Escherichia coli NAD(P)H-dependent quinone oxidoreductase (QOR) of molecular mass 35 kDa. Crystals were grown by the vapour diffusion method in the presence of NADPH and diffract strongly to beyond 2·0 Å. The space group was determined to be P212121 with unit cell dimensions of a=107·44 Å, b=104·06 Å, c=77·45 Å. There is one dimer in the asymmetric unit. © 1994 Academic Press Limited.

引用

页码：501 / 503

页数：3

共 21 条

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