PREPARATION OF EUROPIUM-LABELED DERIVATIVES OF CORTISOL FOR TIME-RESOLVED FLUOROIMMUNOASSAYS

被引：24

作者：

MIKOLA, H

MIETTINEN, P

机构：

[1] Wallac Chemical Laboratories, Turku

来源：

STEROIDS | 1991年 / 56卷 / 01期

关键词：

STEROIDS; CORTISOL; TIME-RESOLVED FLUOROIMMUNOASSAY; EUROPIUM; NONRADIOACTIVE LABELING;

D O I：

10.1016/0039-128X(91)90109-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cortisol 3-(O-carboxymethyl)oxime, 6- and 21-hemisuccinoxycortisol, and cortisol 7-carboxyethyl thioether were synthesized. These carboxyl derivatives were labeled using a described general labeling method with an europium chelate. The labeled steroids were tested in a competitive time-resolved fluoroimmunoassay using antibodies raised against cortisol. Only the site-homologous antigen-antibody pairs underwent immunoreaction and gave satisfactory calibration curves.

引用

页码：17 / 21

页数：5

共 24 条

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