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TRACE DETERMINATION OF DOMOIC ACID IN SEAWATER AND PHYTOPLANKTON BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF THE FLUORENYLMETHOXYCARBONYL (FMOC) DERIVATIVE

被引：149
作者：
POCKLINGTON, R
MILLEY, JE
BATES, SS
BIRD, CJ
DEFREITAS, ASW
QUILLIAM, MA
机构：
[1] NATL RES COUNCIL CANADA,ATLANTIC REG LAB,1411 OXFORD ST,HALIFAX B3H 3Z1,NS,CANADA
[2] FISHERIES & OCEANS CANADA,BEDFORD INST OCEANOG,SCOTIA FUNDY REG,DARTMOUTH B2Y 4A2,NS,CANADA
来源：
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY | 1990年 / 38卷 / 03期
关键词：
Domoic acid; fluorescence detection; FMOC derivatization; neurotoxin; Nitzschia pungens; phytoplankton; reversed-phase HPLC;
D O I：
10.1080/03067319008026940
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method is presented for the trace determination of domoic acid, a neurotoxic amino acid responsible for cases of Amnesic Shellfish Poisoning resulting from the consumption of contaminated shellfish. The method involves pre-column derivatization with 9-fluorenylmethylchloroformate to form the FMOC derivative followed by reversed-phase HPLC with fluorescence detection. The detection limit for domoic acid in seawater and aqueous extracts is 15pg/mL (50 pM) using gradient elution, a 20 μL injection volume, and a 2.1mm I.D. microbore column. Use of dihydrokainic acid as an internal standard improved quantitation. The method was applied to the detection of domoic acid in seawater, in phytoplankton cultures (Nitzschia pungens forma multiseries), and in natural mixed phytoplankton assemblages in estuarine waters. © 1990, Taylor & Francis Group, LLC. All rights reserved.
引用
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页码：351 / 368
页数：18
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