THE MEASUREMENT OF THE ROTENONE-SENSITIVE NADH CYTOCHROME-C REDUCTASE-ACTIVITY IN MITOCHONDRIA ISOLATED FROM MINUTE AMOUNT OF HUMAN SKELETAL-MUSCLE

Mitochondria isolated from minute amounts (100-500 mg) of human skeletal muscle displayed a very high rotenone-resistant NADH cytochrome c reductase activity. Moreover, compared to succinate cytochrome c reductase activity, a low rate of rotenone-sensitive NADH cytochrome c reductase activity was measured when using standard procedures to disrupt mitochondrial membranes. Only a drastic osmotic shock in distillated water as a mean to disrupt mitochondrial membrane was found to strongly increase the actual rate of the rotenone-sensitive activity. This was accompanied by a decrease in the rotenone-insensitive activity. Using such a simple procedure, the NADH cytochrome c reductase was found 70-80% inhibited by retenone and roughly equivalent to 70-85% of the activity of the succinate cytochrome c reductase. © 1990 Academic Press, Inc.