Energy transfer in spectrally inhomogeneous light-harvesting pigment-protein complexes of purple bacteria

被引:96
作者
Hess, S
Akesson, E
Cogdell, RJ
Pullerits, T
Sundstrom, V
机构
[1] LUND UNIV,DEPT CHEM PHYS,S-22100 LUND,SWEDEN
[2] UMEA UNIV,DEPT CHEM PHYS,S-90187 UMEA,SWEDEN
[3] UNIV GLASGOW,DIV BIOCHEM & MOLEC BIOL,GLASGOW G12 8QQ,LANARK,SCOTLAND
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1016/S0006-3495(95)80137-2
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Energy transfer within the peripheral light-harvesting antenna of the purple bacteria Rhodobacter sphaeroides and Rhodopseudomonas palustris was studied by one- and two-color pump-probe absorption spectroscopy with similar to 100-fs tunable pulses at room temperature and at 77 K. The energy transfer from B800 to B850 occurs with a time constant of 0.7 +/- 0.05 ps at room temperature and 1.8 +/- 0.2 ps at 77 K and is similar in both species. Anisotropy measurements suggest a limited but fast B800 <----> B800 transfer time (tau similar to 0.3 ps). This is analyzed as incoherent hopping of the excitation in a system of spectrally inhomogeneous antenna pigment-protein complexes, by a master equation approach, The simulations show that the measured B800 dynamics is well described as energy transfer with a characteristic average nearest-neighbor pairwise transfer time of 0.35 ps among similar to 10 Bchl molecules in a circular arrangement, in good agreement with the recent high-resolution structure of LH2, The possible presence of fast intramolecular relaxation processes within the Bchl a molecule was investigated by measurement of time-resolved difference absorption spectra and kinetics of Bchl a in solution and in low-temperature glasses, From these measurements it is concluded that fast transients observed at room temperature are due mainly to solvation processes, whereas at 77 K predominantly slower (>10-ps) relaxation occurs.
引用
收藏
页码:2211 / 2225
页数:15
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