MODELING AND MEASUREMENT OF THE SPONTANEOUS MUTATION-RATE IN MAMMALIAN-CELLS

被引：48

作者：

ROSSMAN, TG

GONCHAROVA, EI

NADAS, A

机构：

[1] NYU,MED CTR,KAPLAN COMPREHENSIVE CANC CTR,NEW YORK,NY 10016

[2] IBM CORP,THOMAS J WATSON RES CTR,YORKTOWN HTS,NY 10598

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1995年 / 328卷 / 01期

关键词：

MUTAGENESIS; CELL CULTURE; STATISTICS; MATHEMATICAL MODELING;

D O I：

10.1016/0027-5107(94)00190-G

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The study of spontaneous mutation rates in mammalian cells has been hampered by the lack of an alternative to the cumbersome Luria and Delbruck fluctuation test. A brief review of mathematical treatments of spontaneous mutagenesis, along with some of the limitations of the fluctuation test, is presented. A new experimental method and a simple mathematical model for deriving the spontaneous mutation rate are described. Data from the transgenic Chinese hamster G12 cell line growing at two different rates is analyzed according to this model. The results support the concept that, at least for growing cells, the spontaneous mutation rate is independent of the growth rate, and the mutant fraction increases in a linear fashion with the number of generations.

引用

页码：21 / 30

页数：10

共 30 条

[1] STATISTICAL CONCEPTS IN THE THEORY OF BACTERIAL MUTATION [J].