AUTOMATIC SYNTHESIS OF PHOSPHOPEPTIDES BY PHOSPHORYLATION ON THE SOLID-PHASE

被引：60

作者：

DEBONT, HBA ^{[1
]}

VANBOOM, JH ^{[1
]}

LISKAMP, RMJ ^{[1
]}

机构：

[1] GORLAEUS LABS,POB 9502,2300 RA LEIDEN,NETHERLANDS

来源：

TETRAHEDRON LETTERS | 1990年 / 31卷 / 17期

关键词：

D O I：

10.1016/S0040-4039(00)97399-4

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

An efficient automatic synthesis of phosphopeptides on the solid phase is described. Following completion of their synthesis on the solid support, peptides were phosphorylated using N,N-diisopropyl-bis(4-chlorobenzyl)phosphoramidite. After deprotection, pure phosphopeptides containing a phosphorylated serine or threonine were obtained in high yield. © 1990.

引用

页码：2497 / 2500

页数：4

共 31 条

[1]

ARENDT A, 1989, INT J PEPT PROT RES, V33, P486

[2] SIDE-CHAIN PROTECTED N-ALPHA-FLUORENYLMETHOXYCARBONYLAMINO-ACIDS FOR SOLID-PHASE PEPTIDE-SYNTHESIS - N-ALPHA-FLUORENYLMETHOXYCARBONYL-NG-4-METHOXY-2,3,6-TRIMETHYLBENZENESULPHONYL-L-ARGININE [J].