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MONOFUNCTIONAL CHORISMATE MUTASE FROM BACILLUS-SUBTILIS - KINETIC AND C-13 NMR-STUDIES ON THE INTERACTIONS OF THE ENZYME WITH ITS LIGANDS
被引:65
作者
:
GRAY, JV
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV, DEPT CHEM, CAMBRIDGE, MA 02138 USA
GRAY, JV
EREN, D
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV, DEPT CHEM, CAMBRIDGE, MA 02138 USA
EREN, D
KNOWLES, JR
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV, DEPT CHEM, CAMBRIDGE, MA 02138 USA
KNOWLES, JR
机构
:
[1]
HARVARD UNIV, DEPT CHEM, CAMBRIDGE, MA 02138 USA
[2]
HARVARD UNIV, DEPT BIOCHEM, CAMBRIDGE, MA 02138 USA
来源
:
BIOCHEMISTRY
|
1990年
/ 29卷
/ 37期
关键词
:
D O I
:
10.1021/bi00489a051
中图分类号
:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号
:
071010 ;
081704 ;
摘要
:
The interaction of the monofunctional chorismate mutase from Bacillus subtilis with chorismate and prephenate has been studied kinetically and by NMR spectroscopy with 13C specifically labeled substrates. Prephenate dominates the population of enzyme-bound species, and the “off” rate constant (~60 s−1) obtained from line-broadening experiments is close to the value of kcat for chorismate (50 s−1) determined kinetically. The calculated “on” rate constant for prephenate (8 × 105 M−1s−1) is similar to the value of kcat/Km for chorismate (5 × 105 M−1 s−1). The kinetic parameters of the Bacillus mutase are remarkably insensitive to pH over a wide range and display no solvent isotope effect. These results suggest that the enzyme-catalyzed reaction may be encounter controlled (slowed from the diffusion limit by some feature of the enzyme's active site) and that kcat for chorismate is determined by the product off rate. There is now no evidence to suggest that the skeletal rearrangement on the enzyme surface occurs by a pathway other than a pericyclic process. © 1990, American Chemical Society. All rights reserved.
引用
收藏
页码:8872 / 8878
页数:7
相关论文
共 32 条
[1]
SECONDARY TRITIUM ISOTOPE EFFECTS AS PROBES OF THE ENZYMIC AND NON-ENZYMIC CONVERSION OF CHORISMATE TO PREPHENATE
ADDADI, L
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
ADDADI, L
JAFFE, EK
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
JAFFE, EK
KNOWLES, JR
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
KNOWLES, JR
[J].
BIOCHEMISTRY,
1983,
22
(19)
: 4494
-
4501
[2]
TRANSITION-STATE STABILIZATION AND ENZYMIC CATALYSIS - KINETIC AND MOLECULAR-ORBITAL STUDIES OF REARRANGEMENT OF CHORISMATE TO PREPHENATE
ANDREWS, PR
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,AUSTRALIA
ANDREWS, PR
SMITH, GD
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,AUSTRALIA
SMITH, GD
YOUNG, IG
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,AUSTRALIA
YOUNG, IG
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BIOCHEMISTRY,
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ANDREWS, PR
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,ACT,AUSTRALIA
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,ACT,AUSTRALIA
ANDREWS, PR
HEYDE, E
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,ACT,AUSTRALIA
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,ACT,AUSTRALIA
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[Anonymous], 1986, CATALYSIS CHEM ENZYM
[5]
CHORISMATE MUTASE INHIBITORS - SYNTHESIS AND EVALUATION OF SOME POTENTIAL TRANSITION-STATE ANALOGS
BARTLETT, PA
论文数:
0
引用数:
0
h-index:
0
BARTLETT, PA
NAKAGAWA, Y
论文数:
0
引用数:
0
h-index:
0
NAKAGAWA, Y
JOHNSON, CR
论文数:
0
引用数:
0
h-index:
0
JOHNSON, CR
REICH, SH
论文数:
0
引用数:
0
h-index:
0
REICH, SH
LUIS, A
论文数:
0
引用数:
0
h-index:
0
LUIS, A
[J].
JOURNAL OF ORGANIC CHEMISTRY,
1988,
53
(14)
: 3195
-
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POSSIBLE ROLE FOR WATER DISSOCIATION IN THE SLOW BINDING OF PHOSPHORUS-CONTAINING TRANSITION-STATE-ANALOGUE INHIBITORS OF THERMOLYSIN
BARTLETT, PA
论文数:
0
引用数:
0
h-index:
0
BARTLETT, PA
MARLOWE, CK
论文数:
0
引用数:
0
h-index:
0
MARLOWE, CK
[J].
BIOCHEMISTRY,
1987,
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AN INHIBITOR OF CHORISMATE MUTASE RESEMBLING THE TRANSITION-STATE CONFORMATION
BARTLETT, PA
论文数:
0
引用数:
0
h-index:
0
BARTLETT, PA
JOHNSON, CR
论文数:
0
引用数:
0
h-index:
0
JOHNSON, CR
[J].
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,
1985,
107
(25)
: 7792
-
7793
[8]
DIRECT OBSERVATION OF SUBSTRATE DISTORTION BY TRIOSEPHOSPHATE ISOMERASE USING FOURIER-TRANSFORM INFRARED-SPECTROSCOPY
BELASCO, JG
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
BELASCO, JG
KNOWLES, JR
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
KNOWLES, JR
[J].
BIOCHEMISTRY,
1980,
19
(03)
: 472
-
477
[9]
KINETICS OF 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE INHIBITION BY GLYPHOSATE
论文数:
引用数:
h-index:
机构:
BOOCOCK, MR
COGGINS, JR
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV GLASGOW,DEPT BIOCHEM,GLASGOW G12 8QQ,SCOTLAND
UNIV GLASGOW,DEPT BIOCHEM,GLASGOW G12 8QQ,SCOTLAND
COGGINS, JR
[J].
FEBS LETTERS,
1983,
154
(01)
: 127
-
133
[10]
BOVEY FA, 1988, NUCLEAR MAGNETIC RES
←
1
2
3
4
→
共 32 条
[1]
SECONDARY TRITIUM ISOTOPE EFFECTS AS PROBES OF THE ENZYMIC AND NON-ENZYMIC CONVERSION OF CHORISMATE TO PREPHENATE
ADDADI, L
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
ADDADI, L
JAFFE, EK
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
JAFFE, EK
KNOWLES, JR
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
KNOWLES, JR
[J].
BIOCHEMISTRY,
1983,
22
(19)
: 4494
-
4501
[2]
TRANSITION-STATE STABILIZATION AND ENZYMIC CATALYSIS - KINETIC AND MOLECULAR-ORBITAL STUDIES OF REARRANGEMENT OF CHORISMATE TO PREPHENATE
ANDREWS, PR
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,AUSTRALIA
ANDREWS, PR
SMITH, GD
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,AUSTRALIA
SMITH, GD
YOUNG, IG
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,AUSTRALIA
YOUNG, IG
[J].
BIOCHEMISTRY,
1973,
12
(18)
: 3492
-
3498
[3]
COMMON ACTIVE-SITE MODEL FOR CATALYSIS BY CHORISMATE MUTASE-PREPHENATE DEHYDROGENASE
ANDREWS, PR
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,ACT,AUSTRALIA
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,ACT,AUSTRALIA
ANDREWS, PR
HEYDE, E
论文数:
0
引用数:
0
h-index:
0
机构:
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,ACT,AUSTRALIA
AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DEPT BIOCHEM,CANBERRA 2601,ACT,AUSTRALIA
HEYDE, E
[J].
JOURNAL OF THEORETICAL BIOLOGY,
1979,
78
(03)
: 393
-
403
[4]
[Anonymous], 1986, CATALYSIS CHEM ENZYM
[5]
CHORISMATE MUTASE INHIBITORS - SYNTHESIS AND EVALUATION OF SOME POTENTIAL TRANSITION-STATE ANALOGS
BARTLETT, PA
论文数:
0
引用数:
0
h-index:
0
BARTLETT, PA
NAKAGAWA, Y
论文数:
0
引用数:
0
h-index:
0
NAKAGAWA, Y
JOHNSON, CR
论文数:
0
引用数:
0
h-index:
0
JOHNSON, CR
REICH, SH
论文数:
0
引用数:
0
h-index:
0
REICH, SH
LUIS, A
论文数:
0
引用数:
0
h-index:
0
LUIS, A
[J].
JOURNAL OF ORGANIC CHEMISTRY,
1988,
53
(14)
: 3195
-
3210
[6]
POSSIBLE ROLE FOR WATER DISSOCIATION IN THE SLOW BINDING OF PHOSPHORUS-CONTAINING TRANSITION-STATE-ANALOGUE INHIBITORS OF THERMOLYSIN
BARTLETT, PA
论文数:
0
引用数:
0
h-index:
0
BARTLETT, PA
MARLOWE, CK
论文数:
0
引用数:
0
h-index:
0
MARLOWE, CK
[J].
BIOCHEMISTRY,
1987,
26
(26)
: 8553
-
8561
[7]
AN INHIBITOR OF CHORISMATE MUTASE RESEMBLING THE TRANSITION-STATE CONFORMATION
BARTLETT, PA
论文数:
0
引用数:
0
h-index:
0
BARTLETT, PA
JOHNSON, CR
论文数:
0
引用数:
0
h-index:
0
JOHNSON, CR
[J].
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,
1985,
107
(25)
: 7792
-
7793
[8]
DIRECT OBSERVATION OF SUBSTRATE DISTORTION BY TRIOSEPHOSPHATE ISOMERASE USING FOURIER-TRANSFORM INFRARED-SPECTROSCOPY
BELASCO, JG
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
BELASCO, JG
KNOWLES, JR
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
KNOWLES, JR
[J].
BIOCHEMISTRY,
1980,
19
(03)
: 472
-
477
[9]
KINETICS OF 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE INHIBITION BY GLYPHOSATE
论文数:
引用数:
h-index:
机构:
BOOCOCK, MR
COGGINS, JR
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV GLASGOW,DEPT BIOCHEM,GLASGOW G12 8QQ,SCOTLAND
UNIV GLASGOW,DEPT BIOCHEM,GLASGOW G12 8QQ,SCOTLAND
COGGINS, JR
[J].
FEBS LETTERS,
1983,
154
(01)
: 127
-
133
[10]
BOVEY FA, 1988, NUCLEAR MAGNETIC RES
←
1
2
3
4
→