COLD-STABLE MICROTUBULES IN THE CYTOPLASM OF MOUSE EMBRYO FIBROBLASTS

被引：30

作者：

BERSHADSKY, AD ^{[1
]}

GELFAND, VI ^{[1
]}

SVITKINA, TM ^{[1
]}

TINT, IS ^{[1
]}

机构：

[1] ACAD MED SCI USSR,CANC RES CTR,MOSCOW 109801,USSR

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1979年 / 3卷 / 01期

关键词：

D O I：

10.1016/0309-1651(79)90067-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C. © 1979.

引用

页码：45 / 50

页数：6

共 9 条

[1]

BEHNKE O, 1967, J CELL SCI, V2, P169

[2]

BERSHADSKY AD, CELL BIOL INT REPORT

[3] COLD-LABILE AND COLD-STABLE MICROTUBULES IN MITOTIC SPINDLE OF MAMMALIAN-CELLS [J].