APPLICATION OF FLOW-INJECTION ANALYSIS IN THE ONLINE MONITORING OF SUGARS, LACTIC-ACID, PROTEIN AND BIOMASS DURING LACTIC-ACID FERMENTATIONS

被引:53
作者
NIELSEN, J [1 ]
NIKOLAJSEN, K [1 ]
BENTHIN, S [1 ]
VILLADSEN, J [1 ]
机构
[1] NOVO NORDISK AS,DK-2880 BAGSVAERD,DENMARK
关键词
Biomass; Enzyme reactor; Fermentation; Lactic acid; Protein; Sugars;
D O I
10.1016/S0003-2670(00)83914-8
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A laboratory system for the on-line monitoring of important lactic acid fermentation variables is described. The system contains flow-injection analysers for glucose, lactose, galactose, lactate and protein and a continuous-flow analyser for the biomass concentration. The sugar and lactate analysers are based on enzymatic reactions involving oxidases followed by chemiluminescence detection of the hydrogen peroxide formed. The protein analyser is based on the biuret reaction. The system has been used to monitor many fermentation experiments, and some results are presented as examples. © 1990.
引用
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页码:165 / 175
页数:11
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