ISOLATION OF ROUGH AND SMOOTH MICROSOMES FROM RAT LIVER BY MEANS OF A COMMERCIALLY AVAILABLE CENTRIFUGE

Cation-containing discontinuous sucrose gradients are commonly employed for the subfractionation of liver microsomes in various biochemical investigations. The procedure was originally described for the 60 rotor of the Christ Omega I centrifuge, but in most laboratories the isolation is performed by using the No. 30 or 40 rotors of Spinco centrifuges. It is point d out in this paper that a high cross-contamination occurs when fractionation is performed with these Spinco rotors. However, good separation with a high yield can be obtained with the Spinco centrifuge if the 40.2 rotor and the procedure that is described are employed. © 1969.