EFFECTS OF DIETARY ADMINISTRATION OF CHENODEOXYCHOLIC ACID ON N-METHYL-N-NITROSOUREA-INDUCED COLON CANCER IN RATS

被引:32
作者
SARWAL, AN
COHEN, BI
RAICHT, RF
TAKAHASHI, M
FAZZINI, E
机构
[1] CITY NEW YORK INC,PUBL HLTH RES INST,NEW YORK,NY 10016
[2] MANHATTAN VET ADM HOSP,NEW YORK,NY 10010
关键词
Chenodeoxycholic acid; Colon cancer induction; Fecal bile acid; Neutral sterol;
D O I
10.1016/0005-2760(79)90238-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This experiment examines the effects of chenodeoxycholic acid feeding on tumor incidence in rats treated with N-methyl-N-nitrosourea. Group 1 rats received control chow, group 2 control chow plus chenodeoxycholic acid (0.2%), group 3 control chow and N-methyl-N-nitrosourea, and group 4 control chow plus chenodeoxycholic acid (0.2%) and N-methyl-N-nitrosourea. After 28 weeks pathological and histological examination revealed no tumors in group 1 and group 2 rats. The N-methyl-N-nitrosourea group (group 3) had a tumor incidence of 49% (1.1 tumors/all animals) compared to the N-methyl-N-nitrosourea and chenodeoxycholic acid group (group 4) in which 62% of the animals had tumors (1.1 tumors/all animals). The number of tumors per tumor-bearing animal was slightly lower in group 4. The tumors were primarily adenomas, but 3 carcinomas in situ and 9 invasive carcinomas were also detected. Fecal analyses after 28 weeks showed a slight increase in neutral sterols in the N-methyl-N-nitrosourea group compared to controls (2.49 vs. 1.52 mg/g, respectively). In addition, fecal neutral sterols were slightly elevated in the chenodeoxycholic acid fed rats (groups 2 and 4). Conversion of cholesterol to coprostanol was similar in all groups. Total fecal bile acids were higher in chenodeoxycholic acid fed rats compared to control chow fed rats (groups 2 and 4 vs. groups 1 and 3). Major fecal bile acids in the chenodeoxycholic fed rats were chenodeoxycholic acid, lithocholic acid, α-muricholic acid, β-muricholic acid and ω-muricholic acid. The ability of rats to 6-hydroxylate and 7-hydroxylate bile acids may protect them from the increased tumor incidence observed in other carcinogen-bile acid experiments. © 1979.
引用
收藏
页码:423 / 432
页数:10
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