ELECTROPHORETIC TRANSPORT OF SOLUTES IN AQUEOUS 2-PHASE SYSTEMS

被引：25

作者：

LEVINE, ML

BIER, M

机构：

[1] Center for Separation Science, Department of Chemical Engineering, University of Arizona, Tucson, Arizona

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 08期

关键词：

D O I：

10.1002/elps.1150110804

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrophoretic transport of proteins across the interface between the phases of an aqueous polymer two‐phase system can be greatly impeded in comparison with transport within the individual phase. This effect can be controlled by modifying the affinity of the protein for a phase by suitable manipulations of such variables as pH. The effect is not caused by differences in the electrophoretic velocity between the two phases, nor by large changes in pH at the interface. An analogy exists between this phenomenon and the related subject of diffusion of electrolytes across the phase interface. Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：605 / 611

页数：7

共 23 条

[1]

Albertsson P., 1986, PARTITION CELL PARTI

[2] PARTICLE FRACTIONATION IN LIQUID 2-PHASE SYSTEMS - THE COMPOSITION OF SOME PHASE SYSTEMS AND THE BEHAVIOUR OF SOME MODEL PARTICLES IN THEM APPLICATION TO THE ISOLATION OF CELL WALLS FROM MICROORGANISMS [J].