QUANTITATION OF DNA AND RNA IN CRUDE TISSUE-EXTRACTS BY FLOW-INJECTION ANALYSIS

被引：36

作者：

CALDARONE, EM

BUCKLEY, LJ

机构：

[1] National Oceanic and Atmospheric Administration, National Marine Fisheries Service, Northeast Fisheries Center, Narragansett

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 199卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90281-W

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An automated two-dye flow injection analysis system to quantitate DNA and RNA in curde extracts of tissues is described. The method uses the fluorochrome dyes ethidium bromide and Hoechst 33258. DNA concentration is determined directly from its fluorescence in Hoechst dye. RNA is estimated from fluorescence in ethidium bromide after subtraction of the fluorescence due to DNA. This method has several advantages: a simple extraction procedure, a low detection limit (0.01 μg DNA and 0.10 μg RNA), automation, and a high sample throughput. © 1991.

引用

页码：137 / 141

页数：5

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