DETERMINATION OF LEVAMISOLE IN ANIMAL-TISSUES USING LIQUID-CHROMATOGRAPHY THERMOSPRAY MASS-SPECTROMETRY

被引：19

作者：

CANNAVAN, A ^{[1
]}

BLANCHFLOWER, WJ ^{[1
]}

KENNEDY, DG ^{[1
]}

机构：

[1] DEPT AGR NO IRELAND,DIV VET SCI,BELFAST BT4 3SD,ANTRIM,NORTH IRELAND

来源：

ANALYST | 1995年 / 120卷 / 02期

关键词：

LEVAMISOLE DETERMINATION; ANTHELMINTIC; ANIMAL TISSUE; LIQUID CHROMATOGRAPH THERMOSPRAY MASS SPECTROMETRY;

D O I：

10.1039/an9952000331

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is described for the determination of the anthelmintic levamisole in liver, kidney and muscle using liquid chromatography-thermospray mass spectrometry. Samples are extracted by homogenizing with ethyl acetate, applied to Bakerbond CN solid-phase extraction columns, washed pith chloroform-hexane and eluted with methanol. The extracts are evaporated to dryness under nitrogen and the residues are dissolved in mobile phase and injected into the LC-MS system. The positive M + 1 ion at m/z 205 is monitored. The detection limit for the assay is 5 ng g(-1). Mean recoveries were about 93% for liver, 85% for kidney and 79% for muscle. The assay has been used for statutory testing purposes and for measuring levamisole concentrations in liver,kidney and muscle from sheep dosed with a commercial drench containing levamisole.

引用

页码：331 / 333

页数：3

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