MEASUREMENT OF SCATTERING PROPERTIES OF INDIVIDUAL PARTICLES WITH A SCANNING FLOW CYTOMETER

被引：45

作者：

CHERNYSHEV, AV

PROTS, VI

DOROSHKIN, AA

MALTSEV, VP

机构：

[1] UNIV TURKU,DEPT MED PHYS,SF-20521 TURKU,FINLAND

[2] STRELENKA LTD CO,NOVOSIBIRSK 630090,RUSSIA

[3] NOVOSIBIRSK STATE UNIV,NOVOSIBIRSK 630090,RUSSIA

来源：

APPLIED OPTICS | 1995年 / 34卷 / 27期

关键词：

LIGHT-SCATTERING INDICATRIX; FLOW CYTOMETRY; REFRACTIVE INDEX; PARTICLE SIZING;

D O I：

10.1364/AO.34.006301

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A hydrofocusing head with an optical cuvette has been developed for the flow cytometer to generate complete scatter patterns of single particles at scattering angles ranging from 10 degrees to 120 degrees. The scatter signal has been measured as a function of the angle (a flying indicatrix) by the use of particle motion within a scanning system of the flow cytometer by the use of a single photomultiplier. Scattering data measured with the flow cytometer have been compared with those calculated from Mie theory for latex particles. A calculation algorithm has been used to estimate the size and the refractive index of spherical particles from the scattering data measured.

引用

页码：6301 / 6305

页数：5

共 6 条

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