PURIFICATION OF COMMON BEAN CHLOROPLAST DNA BY DEAE CELLULOSE COLUMN CHROMATOGRAPHY

被引：1

作者：

CARELSE, O

CHETSANGA, CJ

MUBUMBILA, MV

机构：

[1] Department of Biochemistry, University of Zimbabwe, 167 Harare, P.O. Box MP, Mount Pleasant

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 558卷 / 01期

关键词：

D O I：

10.1016/0021-9673(91)80139-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

DEAE cellulose column chromatography has been used for the purification of chloroplast DNA. This technique is more time-efficient than the caesium chloride gradient method. It is a reliable tool and yields a high concentration of double-stranded DNA molecules. The chloroplast DNA obtained after DEAE cellulose chromatography is sensitive to different restriction endonucleases and can be used for DNA fragment cloning and sequencing.

引用

页码：318 / 322

页数：5

共 11 条

[1]

BENSON S A, 1984, Biotechniques, V2, P126

[2]

BOHNERT HJ, 1982, ENCY PLANT PHYSL B, V14, P475

[3]

BURKARD G, 1982, METHODS CHLOROPLAST, P347

[4] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF DNA RESTRICTION FRAGMENTS [J].