PHOSPHOLIPASE-C ACTIVATION BY NEUROTENSIN AND NEUROMEDIN-N IN CHINESE-HAMSTER OVARY CELLS EXPRESSING THE RAT NEUROTENSIN RECEPTOR

被引:60
作者
HERMANS, E [1 ]
MALOTEAUX, JM [1 ]
OCTAVE, JN [1 ]
机构
[1] CATHOLIC UNIV LOUVAIN,NEUROCHIM LAB 1352,10 AVE HIPPOCRATE,B-1200 BRUSSELS,BELGIUM
来源
MOLECULAR BRAIN RESEARCH | 1992年 / 15卷 / 3-4期
关键词
NEUROTENSIN; NEUROMEDIN-N; NEUROTENSIN RECEPTOR; TRANSFECTION; 2ND MESSENGER; INOSITOL PHOSPHATE;
D O I
10.1016/0169-328X(92)90126-V
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The rat neurotensin receptor cDNA sequence was transfected in Chinese hamster ovary cells and cellular clones which stably express the corresponding protein were isolated and characterized. The Scatchard analysis of the specific binding of [H-3]neurotensin indicated a K(d) value of 0.45+/-0.08 nM and a B(max) value of 3.27+/-0.29 pmol/mg of protein. Displacement experiments using peptidic analogs of neurotensin and levocabastine confirmed that the transfected receptor exhibits the binding properties of the neurotensin receptor characterized in the rat brain. Neurotensin stimulated the phosphoinositides hydrolysis in a time- and concentration-dependent manner and this effect was mimicked by neurotensin(8-13) and by neuromedin N. The stimulation of phosphoinositides hydrolysis was not inhibited by pertussis toxin. These results indicate that the transfected cells actively express the rat neurotensin receptor which is functionally coupled to phospholipase C through a pertussis toxin-insensitive GTP-binding protein, and that neuromedin N is able to induce the phosphoinositides turnover by interaction with the neurotensin receptor.
引用
收藏
页码:332 / 338
页数:7
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