THE FET3 GENE OF SACCHAROMYCES-CEREVISIAE ENCODES A MULTICOPPER OXIDASE REQUIRED FOR FERROUS IRON UPTAKE

被引：597

作者：

ASKWITH, C ^{[1
]}

EIDE, D ^{[1
]}

VANHO, A ^{[1
]}

BERNARD, PS ^{[1
]}

LI, LT ^{[1
]}

DAVISKAPLAN, S ^{[1
]}

SIPE, DM ^{[1
]}

KAPLAN, J ^{[1
]}

机构：

[1] UNIV MINNESOTA,SCH MED,DEPT BIOCHEM & MOLEC BIOL,DULUTH,MN 55812

来源：

CELL | 1994年 / 76卷 / 02期

关键词：

D O I：

10.1016/0092-8674(94)90346-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

S. cerevisiae accumulate iron by a process requiring a ferrireductase and a ferrous transporter. We have isolated a mutant, fet3, defective for high affinity Fe(II) uptake. The wild-type FET3 gene was isolated by complementation of the mutant defect. Sequence analysis of the gene revealed the presence of an open reading frame coding for a protein with strong similarity to the family of blue multicopper oxidoreductases. Consistent with the role of copper in iron transport, growth of wild-type cells in copper-deficient media resulted in decreased ferrous iron transport. Addition of copper, but not other transition metals (manganese or zinc), to the assay media resulted in the recovery of Fe(II) transporter activity. We suggest that the catalytic activity of the Fet3 protein is required for cellular iron accumulation.

引用

页码：403 / 410

页数：8

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