A RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR THE MEASUREMENT OF DICLOFENAC IN HUMAN PLASMA

被引：10

作者：

MASON, JL

HOBBS, GJ

机构：

[1] University Department of Anaesthesia, Queens Medical Centre, Clifton Boulevard, Nottingham

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1995年 / 18卷 / 10期

关键词：

D O I：

10.1080/10826079508013959

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high performance liquid chromatographic method is described for the determination of diclofenac in human plasma, using naproxen as the internal standard. This method is simple, rapid and cost-effective. Zinc sulphate (5%) and methanol were used for extraction. Prepared samples were analysed on a nucleosil C18 column using a 35:65 acetonitrile-water phosphate buffered mobile phase (pH 2.8) and ultraviolet detection at 280 nm. The assay was linear in the range 30 ng/ml to 2 mu g/ml, with recovery of extraction ranging from 82 to 97% and a detection limit of 30 ng/ml.

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页码：2045 / 2058

页数：14

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