COMPUTER-ASSISTED DETERMINATION OF THE INNER TEMPERATURE AND PEAK CORRECTION FOR CAPILLARY ELECTROPHORESIS

A statistical algorithm, software package and experimental validation are presented for the buffer temperature and electric current predictions and recalculations of peak migration times. The statistical procedure allows the calculation of the confidence intervals for the buffer temperature and electric current and shows the necessity for an accurate determination of the thermal coefficient of the buffer electric conductivity. The software package implements predictions of the buffer temperature, electric current and migration times for any given voltage and coolant temperature.