ANALYSIS OF POLYMERASE CHAIN-REACTION PRODUCT BY CAPILLARY ELECTROPHORESIS AND ITS APPLICATION TO THE DETECTION OF SINGLE-BASE SUBSTITUTION IN GENES

被引：29

作者：

ARAKAWA, H ^{[1
]}

UETANAKA, K ^{[1
]}

MAEDA, M ^{[1
]}

TSUJI, A ^{[1
]}

机构：

[1] SHOWA UNIV,SCH PHARMACEUT SCI,TOKYO 142,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 664卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)80632-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary gel electrophoresis (CGE) was studied for the direct analysis of polymerase chain reaction (PCR) amplified samples. A low cross-linked polyacrylamide gel (3%T, 0.5%C) was used for CGE with treated and untreated silica capillaries. CGE showed high reproducibility and resolution in the separation of DNA fragments (ca. 100-1000 base pairs) produced by PCR. The CGE system was applied to the detection of an amplification refractory mutation system (ARMS) and PCR-restriction fragment length polymorphism (PCR-RFLP), which are detection methods of single base substitution in genes using PCR. With the CGE system, full automation of PCR product detection is feasible.

引用

页码：89 / 98

页数：10

共 17 条

[1] A SIMPLE METHOD FOR THE PREPARATION OF POLYACRYLAMIDE-GEL FILLED CAPILLARIES FOR HIGH-PERFORMANCE SEPARATION OF POLYNUCLEOTIDES BY USING CAPILLARY GEL-ELECTROPHORESIS [J].