CHIMERIC ENVELOPE GLYCOPROTEINS CONSTRUCTED BETWEEN AMPHOTROPIC AND XENOTROPIC MURINE LEUKEMIA RETROVIRUSES

被引：7

作者：

HAN, L ^{[1
]}

HOFMANN, T ^{[1
]}

CHIANG, Y ^{[1
]}

ANDERSON, WF ^{[1
]}

机构：

[1] NIH,MOLEC HEMATOL BRANCH,BETHESDA,MD 20892

来源：

SOMATIC CELL AND MOLECULAR GENETICS | 1995年 / 21卷 / 03期

关键词：

D O I：

10.1007/BF02254771

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A set of chimeric envelope proteins between amphotropic and xenotropic murine leukemia retroviruses (MuLV), two closely related members in the MuLV family, were constructed The purpose was to examine the regions that could be successfully enchanged between these two similar viral envelope proteins. The data indicate that fully active chimeras can be built when the junction is either at the EcoRI site (amino acid 169) 42 amino acids N-terminal to the polyproline hinge of gp70 (named CH4) or at the ScaI site (aa 593) in the membrane spanning portion of p15E (CH1). However, a chimera at the AflII site (aa 125, CH5) and two in the C-terminal end of gp70 (aa 418, CH2; aa 326, CH3) were inactive. These results, taken together with other data from our laboratory and others, suggest that the entire gp70/p15E structure is sensitive to alterations and that even envelope proteins that are very similar have only a limited ability to exchange sequences.

引用

页码：205 / 214

页数：10

共 23 条

[1] RECEPTOR CHOICE DETERMINANTS IN THE ENVELOPE GLYCOPROTEINS OF AMPHOTROPIC, XENOTROPIC, AND POLYTROPIC MURINE LEUKEMIA VIRUSES [J].