FIBER OPTIC FLUORESCENCE SCANNING IN THIN-LAYER CHROMATOGRAPHY

被引:6
作者
DIAZ, AN
SANCHEZ, FG
机构
[1] Departamento de Quimica Analftica, Facultad de Ciencias, Universidad de Malaga, 29071., Malaga
关键词
D O I
10.1080/10739149408000455
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The effectiveness of fluorometric scanning using a fiber optic to analyze substances adsorbed onto solid surfaces is described. The relative efficiencies of signal capture by different widths of monochromator slits were studied. The signal and signal-to-noise ratios were compared using monochromators or long-pass filters. Accuracy and precision of the measurements were compared with and without substraction of the background signal; the detection limits for fluorescein and several phenothiazine compounds were compared under different experimental conditions to explore the range of effectiveness of the system.
引用
收藏
页码:273 / 281
页数:9
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