DETERMINATION OF LIPASE ACTIVITY IN AOT-ISOOCTANE REVERSED MICELLES

被引：21

作者：

HAN, D

KWON, DY

RHEE, JS

机构：

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1987年 / 51卷 / 02期

关键词：

D O I：

10.1080/00021369.1987.10868020

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

An assay method in a reversed micellar system, which can be better characterized in physicochemical terms than that in an emulsion system, was investigated. The first-order rate constant when [S] .mchlt. Km and the zero-order rate constant when [S] .mchgt. Km for lipase reaction in the system show a linear relationship with enzyme concentration over a twenty-fold range, which is an important criterion for an enzyme assay. Based on the results, a three-point assay of lipase in reversed micelles was established. To ensure the initial rate, the assay time should be limited to the time during which about 4.5% of the substrate is utilized.

引用

页码：615 / 618

页数：4

共 17 条

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