PASSAGE OF [125I]INSULIN ACROSS ISOLATED MESENTERY EFFECT OF ANTI-INSULIN SERUM

The exchange of insulin, inulin, dextran and albumin across isolated rat mesentery is inversely related to molecular weight. The kinetics of the exchange are compatible with the laws of passive diffusion. After a 2-h incubation, 1% of the diffusible insulin is adsorbed on the membrane. An excess of guinea-pig anti-insulin serum prevents the passage of insulin by forming a complex of high molecular weight and by blocking the membrane pathways of insulin diffusion. By contrast, under specific experimental conditions, appropriate amounts of guinea-pig anti-insulin serum can increse the transmesenteric flux of insulin. © 1969.