A 36-KILODALTON CELLULAR TRANSCRIPTION FACTOR MEDIATES AN INDIRECT INTERACTION OF HUMAN T-CELL LEUKEMIA LYMPHOMA VIRUS TYPE-I TAX1 WITH A RESPONSIVE ELEMENT IN THE VIRAL LONG TERMINAL REPEAT

被引：75

作者：

MARRIOTT, SJ ^{[1
]}

LINDHOLM, PF ^{[1
]}

BROWN, KM ^{[1
]}

GITLIN, SD ^{[1
]}

DUVALL, JF ^{[1
]}

RADONOVICH, MF ^{[1
]}

BRADY, JN ^{[1
]}

机构：

[1] NCI, MOLEC VIROL LAB, BETHESDA, MD 20892 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 08期

关键词：

D O I：

10.1128/MCB.10.8.4192

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human T-cell leukemia/lymphoma virus type I (HTLV-I) trans activator, TAX1, interacts indirectly with a TAX1,-responsive element, TRE-2, located at positions -117 to -163 in the viral long terminal repeat. This report describes the characterization of a 36-kilodalton (kDa) protein identified in HeLa nuclear extract which mediates the interaction of TAX1 with TRE-2. Purification of the protein was achieved by zinc chelate chromatography and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The renatured 36-kDa protein bound specifically to a TRE-2 oligonucleotide but not to nonfunctional base substitution mutant probes in a gel retardation assay. Renatured proteins of differing molecular weights were unable to form this complex. In addition, the 36-kDa protein specifically activated transcription from the HTLV-I promoter in vitro. Purified TAX1 protein formed a complex with the TRE-2 oligonucleotide in the presence of the 36-kDa protein, suggesting that indirect interaction of TAX1 with the viral long terminal repeat may be one of the mechanisms by which HTLV-I transcription is regulated.

引用

页码：4192 / 4201

页数：10

共 70 条

[1] HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I AND TYPE-2 EXHIBIT DIFFERENT DNASE I PROTECTION PATTERNS [J].