COMPARISON OF RADIOIMMUNOASSAY AND CAPILLARY GAS-CHROMATOGRAPHY IN THE ANALYSIS OF L-HYOSCYAMINE FROM PLANT-MATERIAL

被引：6

作者：

MARTINSEN, A

NAARANLAHTI, T

TURKIA, ML

LEHTOLA, T

OKSANEN, J

YLINEN, M

机构：

[1] Department of Pharmaceutical Chemistry, University of Kuopio, Kuopio, 7021

[2] University Pharmacy, Turku, 20100

[3] Department of Environmental Hygiene, University of Kuopio, Kuopio, 7021

[4] University Pharmacy, Kuopio, 70100

来源：

PHYTOCHEMICAL ANALYSIS | 1991年 / 2卷 / 04期

关键词：

HYOSCYAMINE; CAPILLARY GAS CHROMATOGRAPHY; RADIOIMMUNOASSAY; SOLANACEAE; ATROPA-BELLADONNA;

D O I：

10.1002/pca.2800020404

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

l-Hyoscyamine in the roots of Atropa belladonna L. was assayed by capillary gas chromatography (CGC). When the extraction and analysis were evaluated using a radioimmunoassay (RIA) established for l-hyoscyamine, the two methods were found to be positively and significantly correlated. Important characteristics of CGC and RIA methods in tropane alkaloid analysis of plant material are discussed. A prominent cross-reaction for apoatropine, a constituent of A. belladonna roots and leaves, was found in this study. This cross-reaction has not been investigated in other studies of hyoscyamine RIA.

引用

页码：163 / 166

页数：4

共 18 条

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