MEASUREMENT OF BINDING-KINETICS USING THE GEL-ELECTROPHORESIS MOBILITY SHIFT ASSAY

被引：18

作者：

GERSTLE, JT

FRIED, MG

机构：

[1] PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT BIOL CHEM,HERSHEY,PA 17033

[2] PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT SURG,HERSHEY,PA 17033

来源：

ELECTROPHORESIS | 1993年 / 14卷 / 08期

关键词：

D O I：

10.1002/elps.11501401115

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The gel electrophoresis mobility shift assay is a technique for the qualitative and quantitative analysis of protein-DNA complexes. The ability to resolve reactants, reaction intermediates and products makes this method particularly well-suited for the measurement of the assembly and dissociation rates of protein-nucleic acid complexes. Here we identify conditions that must be met and variations of the technique that are useful for the measurement of reaction rates.

引用

页码：725 / 731

页数：7

共 30 条

[1] AUTO-REGULATION OF THE ESCHERICHIA-COLI CRP GENE - CRP IS A TRANSCRIPTIONAL REPRESSOR FOR ITS OWN GENE
AIBA, H
[J]. CELL, 1983, 32 (01) : 141 - 149
[2] ANDERSON WB, 1971, J BIOL CHEM, V246, P5929
[3] RAPID HIGH-YIELD PURIFICATION PROCEDURE FOR CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE RECEPTOR PROTEIN FROM ESCHERICHIA-COLI
BOONE, T
WILCOX, G
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 541 (04) : 528 - 534
[4] BUCKLE M, 1991, METHOD ENZYMOL, V208, P236
[5] MOLECULAR-PARAMETERS CHARACTERIZING INTERACTION OF ESCHERICHIA-COLI LAC REPRESSOR WITH NON-OPERATOR DNA AND INDUCER
BUTLER, AP
REVZIN, A
VONHIPPEL, PH
[J]. BIOCHEMISTRY, 1977, 16 (22) : 4757 - 4768
[6] CAEY J, 1991, METHOD ENZYMOL, V208, P103
[7] CANN JR, 1989, J BIOL CHEM, V264, P17032
[8] GEL RETARDATION AT LOW PH RESOLVES TRP REPRESSOR-DNA COMPLEXES FOR QUANTITATIVE STUDY
CAREY, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (04) : 975 - 979
[9] STUDIES OF DNA-PROTEIN INTERACTIONS BY GEL-ELECTROPHORESIS
CEGLAREK, JA
REVZIN, A
[J]. ELECTROPHORESIS, 1989, 10 (5-6) : 360 - 365
[10] GEL-ELECTROPHORESIS OF PROTEIN DNA COMPLEXES
CROTHERS, DM
[J]. NATURE, 1987, 325 (6103) : 464 - 465

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