NADPH CYTOCHROME-P-450 REDUCTASE IN RAT, MOUSE AND HUMAN BRAIN

被引：39

作者：

RAVINDRANATH, V ^{[1
]}

ANANDATHEERTHAVARADA, HK ^{[1
]}

SHANKAR, SK ^{[1
]}

机构：

[1] NATL INST MENTAL HLTH & NEUROSCI,DEPT NEUROPATHOL,BANGALORE 560029,KARNATAKA,INDIA

来源：

BIOCHEMICAL PHARMACOLOGY | 1990年 / 39卷 / 06期

关键词：

D O I：

10.1016/0006-2952(90)90279-T

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

NADPH cytochrome P-450 reductase (P-450 reductase), an essential component of the cytochrome P-450 mono-oxygenase system, has been estimated in rat and mouse brain, and seven human brains obtained at autopsy. The ratio of cytochrome P-450 to P-450 reductase is lower in the rat and mouse brains (2.5-4.0) as compared to the respective livers (10.0-11.0). The rat and mouse brain P-450 reductase were immunologically similar to the rat liver P-450 reductase as examined by immunochemical inhibition, Ouchterlony double diffusion and immunoblot. The antisera to rat liver P-450 reductase inhibited rat brain aminopyrine N-demethylase activity to the same extent as NADPH cytochrome c reductase, suggesting that the level of P-450 reductase controls the rate of this cytochrome P-450 mediated activity. The human brain NADPH cytochrome c reductase exhibited regional variation, maximal activity being observed in the brain stem region. Immunochemical inhibition and immunoblot studies revealed immunological cross-reactivity between rat liver reductase and human brain medulla, while none was observed in cortex or cerebellum. Immunocytochernical studies on human brain medulla using antisera to rat liver P-450 reductase indicated localization of the P-450 reductase in neuronal cell body. © 1990.

引用

页码：1013 / 1018

页数：6

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