DIAGNOSIS OF ACUTE TYPHUS INFECTION USING THE POLYMERASE CHAIN-REACTION

被引：70

作者：

CARL, M ^{[1
]}

TIBBS, CW ^{[1
]}

DOBSON, ME ^{[1
]}

PAPARELLO, S ^{[1
]}

DASCH, GA ^{[1
]}

机构：

[1] NATL NAVAL MED CTR, BETHESDA, MD 20814 USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 1990年 / 161卷 / 04期

关键词：

D O I：

10.1093/infdis/161.4.791

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The first use of the polymerase chain reaction (PCR) for the diagnosis of an acute rickettsial infection is described. A primer pair derived from the 17-kDa antigen sequence of Rickettsia rickettsii gave specific amplification of a 434-base pair DNA fragment from the genome of Rocky Mountain spotted fever and endemic and epidemic typhus. The assay could detect as few as 30 rickettsiae. Detection of PCR-amplified DNA with a nonradioactive DNA probe confirmed an acute infection with Rickettsia prowazekii. © 1990, University of Chicago. All rights reserved.

引用

页码：791 / 793

页数：3

共 15 条

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