EFFICIENT INCORPORATION OF GALACTOSE INTO LIPOPOLYSACCHARIDE BY ESCHERICHIA-COLI K-12 STRAINS WITH POLAR GALE MUTATIONS

被引：13

作者：

SCHNAITMAN, CA ^{[1
]}

AUSTIN, EA ^{[1
]}

机构：

[1] UNIV VIRGINIA,SCH MED,DEPT MICROBIOL,CHARLOTTESVILLE,VA 22908

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 09期

关键词：

D O I：

10.1128/jb.172.9.5511-5513.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Efficient incorporation of exogenous galactose into lipopolysaccharide was observed in a strain with a galE::Tn10 insertion and a strain with a deletion of galOPE. No incorporation was observed in a strain with a longer (galETK) deletion, indicating that incorporation into the galE mutants was due to a low level of expression of galTK from internal or fortuitous promoters. These galE mutants provided a convenient and specific way to radiolabel lipopolysaccharide.

引用

页码：5511 / 5513

页数：3

共 17 条

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