HIGH-FREQUENCY OF MUTATION TO TUBERCIDIN RESISTANCE IN CHO CELLS

被引：33

作者：

RABIN, MS

GOTTESMAN, MM

机构：

[1] Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, 20205, Maryland

来源：

SOMATIC CELL GENETICS | 1979年 / 5卷 / 05期

关键词：

D O I：

10.1007/BF01542695

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The acquisition of high-level resistance to tubercidin (an adenosine analog) in CHO cells occurs in a single step at high frequency (10-3 to 10-4) without mutagenesis. Analysis of a large number of independent mutants by a fluctuation test (Luria and Delbruck, 1943) indicates that they arise independently of the selection medium and all fall into the same complementation group. All mutants tested lack detectable adenosine kinase activity. An analysis of hybrids between mutant and wild-type cells indicates that resistance to tubercidin is a recessive marker which segregates as would be expected if it were a haploid locus in the parental CHO cell. Resistance to tubercidin is not linked to the X chromosome in CHO cells and appears to occur at much lower frequency in primary Chinese hamster cells and other cultured cell lines. © 1979 Plenum Publishing Corporation.

引用

页码：571 / 583

页数：13

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