CLONING AND INTRACELLULAR-LOCALIZATION OF THE U2 SMALL NUCLEAR RIBONUCLEOPROTEIN AUXILIARY FACTOR SMALL SUBUNIT

被引：188

作者：

ZHANG, M ^{[1
]}

ZAMORE, PD ^{[1
]}

CARMOFONSECA, M ^{[1
]}

LAMOND, AI ^{[1
]}

GREEN, MR ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB, W-6900 HEIDELBERG, GERMANY

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 18期

关键词：

SPLICING; SPLICEOSOME; RNA BINDING; ARGININE; SERINE RICH; COILED BODY;

D O I：

10.1073/pnas.89.18.8769

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35. U2AF35 contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF65 and U2AF35 interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF35, we show that the protein has the intracellular distribution characteristic of U2AF65. Both U2AF65 and U2AF35 are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903.

引用

页码：8769 / 8773

页数：5

共 33 条

[1] THE SEX-DETERMINING GENE TRA-2 OF DROSOPHILA ENCODES A PUTATIVE RNA-BINDING PROTEIN [J].