AUTOMATED METAPHASE FINDING - AN ASSESSMENT OF THE EFFICIENCY OF THE METAFER2 SYSTEM IN A ROUTINE MUTAGENICITY ASSAY

被引：16

作者：

HUBER, R ^{[1
]}

KULKA, U ^{[1
]}

LORCH, T ^{[1
]}

BRASELMANN, H ^{[1
]}

BAUCHINGER, M ^{[1
]}

机构：

[1] METASYST,D-69207 SANDHAUSEN,GERMANY

来源：

MUTATION RESEARCH-ENVIRONMENTAL MUTAGENESIS AND RELATED SUBJECTS | 1995年 / 334卷 / 01期

关键词：

AUTOMATED METAPHASE FINDING; IMAGE ANALYSIS; CHROMOSOME ABERRATIONS; SISTER-CHROMATID EXCHANGE; METAFER2;

D O I：

10.1016/0165-1161(95)90035-7

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The efficiency of the automated metaphase finding system METAFER2 is assessed in a routine mutagenicity assay using an aneuploid rat liver cell line treated with various promutagens. Data sets generated by automated and manual selection of metaphases are compared. It is demonstrated that METAFER2 routinely allows an efficient automatic identification of metaphases not only in lymphocyte preparations, but also in preparations from mammalian cell lines with varying chromosome numbers. Although larger slide areas are required for automated compared to manual metaphase scanning, the automatic system is faster by a factor of about 5. The interactive visual elimination of metaphases of insufficient quality is an easy and fast procedure. METAFER2 allows an unbiased selection of metaphases irrespective of their appearance as homogeneously stained first or harlequin-stained second division cells. Random selection of metaphases is neither influenced by various structural chromosome changes nor by increased frequencies of sister-chromatid exchanges.

引用

页码：97 / 102

页数：6

共 10 条

[1] AN ASSESSMENT OF THE METAPHASE FINDING CAPABILITY OF THE CYTOSCAN-110 [J].