RAPID PROTEIN EXTRACTION FROM SACCHAROMYCES-CEREVISIAE

被引：137

作者：

HORVATH, A ^{[1
]}

RIEZMAN, H ^{[1
]}

机构：

[1] UNIV BASEL, BIOZENTRUM, DEPT BIOCHEM, CH-4056 BASEL, SWITZERLAND

来源：

YEAST | 1994年 / 10卷 / 10期

关键词：

YEAST; WESTERN BLOTTING; LYSIS; SDS-PAGE;

D O I：

10.1002/yea.320101007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed and evaluated an easy and rapid method for extraction of proteins from yeast cells for sodium dodecyl sulphate (SDS)-gel electrophoresis and Western blotting. The procedure comprises a centrifugation step to harvest the cells, addition of a sample buffer and heating, then another centrifugation step before applying the extracted proteins found in the supernatant to an SDS gel. It is applicable to the study of large numbers of samples in 1 day. This procedure is easier, quicker, and as efficient as procedures using base and 2-mercaptoethanol, but somewhat less efficient than lysis with glass beads under certain conditions.

引用

页码：1305 / 1310

页数：6

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