MULTILABELING OF FERROCENES TO A GLUCOSE-OXIDASE DIGOXIN CONJUGATE FOR THE DEVELOPMENT OF A HOMOGENEOUS ELECTROENZYMATIC IMMUNOASSAY

被引：45

作者：

SUZAWA, T ^{[1
]}

IKARIYAMA, Y ^{[1
]}

AIZAWA, M ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,FAC BIOSCI & BIOTECHNOL,DEPT BIOENGN,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 22期

关键词：

D O I：

10.1021/ac00094a008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new homogeneous electroenzymatic immunoassay was developed to determine antibody concentration using glucose oxidase and ferrocene as enzymatic and electrochemical amplifier, respectively. Digoxin (Dig)-conjugated glucose oxidase (GOx) was modified with ferrocene (Fec) to form Fec-GOx-Dig conjugate. After immunocomplex formation between the Fec-GOx-Dig conjugate and the anti-digoxin antibody, the complex underwent less electrochemical reaction due to the steric hindrance of the antibody. The ferrocene multilabeled conjugate was provided for the determination of anti-digoxin antibody. Since the strategy taken here is based on a combined effect of GOx and ferrocene, i.e., enzymatic amplification by GOx and electrochemical amplification by multilabeled ferrocenes, the antibody concentration was determined in the range from 1/50 to 1/500 dilution.

引用

页码：3889 / 3894

页数：6

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