PARTITION ASSAY FOR EPOXIDE HYDRASES ACTING ON INSECT JUVENILE HORMONE AND AN EPOXIDE-CONTAINING JUVENOID

被引：48

作者：

MUMBY, SM

HAMMOCK, BD

机构：

[1] Division of Toxicology and Physiology, Department of Entomology, University of California, Riverside

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 92卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90619-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay for epoxide hydrase activity is described based on the difference in solubility between the epoxide substrate and its corresponding diol. The assay conditions for insect juvenile hormone I and an epoxide-containing juvenoid have been elucidated. In each case, the epoxide partitions into an isooctane phase while the diol partitions largely into an aqueous methanol phase. The assay is faster than chromatographic assays while showing similar or superior precision. © 1979.

引用

页码：16 / 21

页数：6

共 22 条

[1] INSECT EPOXIDE HYDRASE INHIBITION BY JUVENILE-HORMONE ANALOGS AND METABOLIC INHIBITORS [J].