DETECTION OF THE INVIVO CONVERSION OF 2-PYRROLIDINONE TO GAMMA-AMINOBUTYRIC ACID IN MOUSE-BRAIN

被引：33

作者：

CALLERY, PS

STOGNIEW, M

GEELHAAR, LA

机构：

[1] Department of Medicinal Chemistry, School of Pharmacy, University of Maryland, Baltimore, Maryland

来源：

BIOMEDICAL MASS SPECTROMETRY | 1979年 / 6卷 / 01期

关键词：

D O I：

10.1002/bms.1200060106

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Labeled γ‐aminobutyric acid was detected in mouse brain following intravenous injections of deuterium labeled 2‐pyrrolidinone. [2H6]Pyrrolidinone was prepared by the reduction of [2H4]succinimide with lithium aluminum deuteride. Quantification was accomplished by a gas chromatography mass spectrometry assay method. γ‐Aminobutyric acid and internal standard, 5‐aminovaleric acid, were converted to volatile derivatives by treatment with N,N‐dimethylformamide dimethyl acetal. Quantitative estimates were derived from peak area measurements obtained from monitoring the parent ions of the γ‐aminobutyric acid and internal standard derivatives by repetitive scanning during the GC run. The conversion of pyrrolidinone to γ‐aminobutyric acid may provide a method for labeling central γ‐aminobutyric acid pools. Copyright © 1979 Heyden & Son Ltd

引用

页码：23 / 26

页数：4

共 18 条

[1] MASS FRAGMENTOGRAPHIC QUANTITATION OF GLUTAMIC-ACID AND GAMMA-AMINOBUTYRIC ACID IN CEREBELLAR NUCLEI AND SYMPATHETIC-GANGLIA OF RATS [J].