STABILIZATION, PURIFICATION AND CRYSTALLIZATION OF CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE FROM BOVINE HEART

被引：34

作者：

OKUNO, S ^{[1
]}

FUJISAWA, H ^{[1
]}

机构：

[1] ASAHIKAWA MED COLL, DEPT BIOCHEM, ASAHIKAWA 07811, JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1038卷 / 02期

关键词：

(Bovine heart); cAMP-dependent protein kinase; Catalytic subunit; Crystallization; Purification; Stabilization;

D O I：

10.1016/0167-4838(90)90206-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The catalytic subunit of cAMP-dependent protein kinase purified from bovine heart was significantly stabilized by non-ionic detergents. Freezing and thawing did not cause a significant decrease in the enzyme activity in the presence of 0.1% Tween 80. A very convenient method for purification and crystallization of the catalytic subunit was also described. © 1990.

引用

页码：204 / 208

页数：5

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