MASS FRAGMENTOGRAPHIC ANALYSIS OF JUVENILE HORMONE-1 LEVELS DURING THE LAST LARVAL INSTAR OF PIERIS-BRASSICAE

被引：43

作者：

MAUCHAMP, B ^{[1
]}

LAFONT, R ^{[1
]}

JOURDAIN, D ^{[1
]}

机构：

[1] INST NATL AGRON, F-78850 THIVERVAL GRIGNON, FRANCE

来源：

JOURNAL OF INSECT PHYSIOLOGY | 1979年 / 25卷 / 07期

关键词：

Juvenile hormones; mass spectrometry; Pieris brassicae;

D O I：

10.1016/0022-1910(79)90068-4

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

A method is presented here for routine analyses of juvenile hormone levels using coupled gas-liquid chromatography-mass fragmentography with chemical ionization. This method is sensitive and highly specific, but needs complex equipment. It has been used for an analysis of Pieris brassicae haemolymph during the last larval instar. Only juvenile hormone I was detected at significant levels (between less than 100 pg/ml and 6 ng/ml). Juvenile hormone I variations are far more complex than expected and show a discrete peak (1 ng/ml) at the time when pupal programming takes place. This finding is consistent with the 'classical scheme', where pupal moult occurs in the presence of reduced juvenile hormone levels. © 1979.

引用

页码：545 / 550

页数：6

共 38 条

[1] SIMPLE AND SENSITIVE RADIOIMMUNOASSAY OF INSECT JUVENILE HORMONE USING AN IODINATED TRACER [J].