UTILIZATION OF HUMAN CULTURED EPIDERMAL-KERATINOCYTES - IRREVERSIBILITY OF THE INHIBITION OF PROLIFERATION INDUCED IN STORED DETACHED CULTURES

被引:7
作者
BOUCHER, F [1 ]
POUMAY, Y [1 ]
DEGEN, A [1 ]
PAYE, M [1 ]
LELOUP, R [1 ]
机构
[1] FAC UNIV NOTRE DAME LA PAIX,DEPT HIST EMBRYOL,RUE BRUXELLES 61,B-5000 NAMUR,BELGIUM
关键词
D O I
10.1016/0305-4179(91)90105-P
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
In order to look for the best conditions required for the utilization of cultured epithelium in the treatment of burn wounds, some experiments have been performed studying the storage of dispase-detached cultures. The viability of keratinocytes and, after trypsin dissociation of the cultures, the adhesion and proliferative potential of living keratinocytes were measured. Our laboratory investigations suggest that the storage period of detached cultures has to be kept as short as possible to preserve the keratinocytes' growth potential.
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页码:205 / 208
页数:4
相关论文
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