FLUORESCENCE POLARIZATION IMMUNOASSAY FOR CYCLOSPORINE-A DETERMINATION IN WHOLE-BLOOD

被引：4

作者：

PLEBANI, M ^{[1
]}

PALEARI, CD ^{[1
]}

MASIERO, M ^{[1
]}

FAGGIAN, D ^{[1
]}

BURLINA, A ^{[1
]}

机构：

[1] UNIV PADUA,DEPT CLIN BIOCHEM,CENT LAB,VIA GUISTINIANI 2,I-35128 PADUA,ITALY

来源：

THERAPEUTIC DRUG MONITORING | 1990年 / 12卷 / 03期

关键词：

Cyclosporine a; Fluorescence polarization immunoassay; High-performance liquid chromatography; Radioimmunoassay;

D O I：

10.1097/00007691-199005000-00013

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A fluorescence polarization immunoassay (FPIA) for cyclosporine A (CsA) determination in blood was evaluated. The method demonstrated a good reproducibility both in within-run and between-run assays. The linearity range was 35-990 μg/L. Comparison of measurements in patients’ whole blood with radioimmunoassay results showed higher estimates by the FPIA despite the good correlation coefficient. Finally, a poor agreement was observed between the evaluated method and the high-performance liquid chromatographic technique for CsA determination. © 1990 Raven Press, Ltd., New York.

引用

页码：284 / 287

页数：4

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